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TitleStrategies to Suppress Hydrogen-Consuming Microorganisms Affect Macro and Micro Scale Structure and Microbiology of Granular Sludge
Author(s)Abreu, A. A.
Alves, J. I.
Pereira, M. A.
Sousa, D. Z.
Alves, M. M.
dark fermentation
image analysis
bacterial communities
EGSB reactors
Issue dateAug-2011
PublisherJohn Wiley and Sons
JournalBiotechnology and Bioengineering
Abstract(s)Treatment of anaerobic granules with heat and two chemical treatments, contacting with 2-bromoethanesulfonate (BES) and with BESþChloroform, were applied to suppress hydrogen-consuming microorganisms. Three mesophilic expanded granular sludge bed (EGSB) reactors— RHeat, RBES, and RBESþChlo—were inoculated with the treated sludges and fed with synthetic sugar-based wastewater (5 gCOD L 1, HRT 20–12 h). Morphological integrity of granules and bacterial communities were assessed by quantitative image analysis and 16S rRNA gene based techniques, respectively. Hydrogen production in RHeat was under 300mLH2 L 1 day 1, with a transient peak of 1,000 mLH2 L 1 day 1 after decreasing HRT. In RBESþChlo hydrogen production rate did not exceed 300mLH2 L 1 day 1 and there was granule fragmentation, release of free filaments from aggregates, and decrease of granule density. In RBES, there was an initial period with unstable hydrogen production, but a pulse of BES triggered its production rate to 700 200mLH2 L 1 day 1. This strategy did not affect granules structure significantly. Bacteria branching within Clostridiaceae and Ruminococcaceae were present in this sludge. This work demonstrates that, methods applied to suppress H2-consuming microorganisms can cause changes in the macro- and microstructure of granular sludge, which can be incompatible with the operation of high-rate reactors.
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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