Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/17306

TitleProteolytic enzyme engineering : a tool for wool
Author(s)Araújo, Rita
Silva, Carla Manuela Pereira Marinho da
Machado, Raul
Casal, Margarida
Cunha, A. M.
Rodríguez-Cabello, José Carlos
Paulo, Artur Cavaco
Issue date2009
PublisherACS Publications
JournalBiomacromolecules
Abstract(s)One of the goals of protein engineering is to tailor the structure of enzymes to optimize industrial bioprocesses. In the present work, we present the construction of a novel high molecular weight subtilisin, based on the fusion of the DNA sequences coding for Bacillus subtilis prosubtilisin E and for an elastin-like polymer (ELP). The resulting fusion protein was biologically produced in Escherichia coli, purified and used for wool finishing assays. When compared to the commercial protease Esperase, the recombinant subtilisinE-VPAVG220 activity was restricted to the cuticle of wool, allowing a significant reduction of pilling, weight loss and tensile strength loss of wool fibers. Here we report, for the first time, the microbial production of a functionalized high molecular weight protease for controlled enzymatic hydrolysis of wool surface. This original process overcomes the unrestrained diffusion and extended fiber damage which are the major obstacles for the use of proteases for wool finishing applications.
TypeArticle
URIhttp://hdl.handle.net/1822/17306
DOI10.1021/bm9002943
ISSN1525-7797
Peer-Reviewedyes
AccessOpen access
Appears in Collections:DET/2C2T - Artigos em revistas internacionais com arbitragem científica

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