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TitleApplication of an electronic tongue to detect gliadins in gluten-free foods
Author(s)Peres, A. M.
Dias, Luís G.
Veloso, Ana C. A.
Meirinho, Sofia G.
Morais, J. Sá
Machado, A. A. S. C.
Issue date2010
Abstract(s)Celiac disease is an autoimmune-mediated disorder triggered in genetically susceptible individuals by the ingestion of some gluten proteins, namely gliadins. To prevent inadvertent gluten consumption, the Commission Regulation (EC) Nº41/2009 will implement labelling foods as “gluten-free” or “low-gluten content”[1]. The feasibility of an all-solid-state potentiometric electronic tongue to detect the contamination of “gluten-free” foodstuffs with gliadins was evaluated. The device was constituted by 36 cross-sensibility lipo/polymeric membranes and its performance was assessed using food ethanolic extracts (Figure 1). A semi-quantitative linear discriminant (LDA) model was established based on the signal patterns of 11 polymeric membranes. The model was able to distinguish between “Gluten-free” extracts (<10 ppm gliadins), “Low-Gluten content” extracts (20-50 ppm gliadins) and “Gluten-containing” extracts (>50 ppm gliadins), with sensibilities of 100% and 77% for the original grouped cases and “leaving one-out” cross-validation, respectively (Figure 2). The device sensitivity was towards 1-2 ppm. The device was also applied to discriminate between “gluten-free” and “glutencontaining” foodstuffs (flours, baby milked flours, breads, cookies and breakfast cereals), being the label information checked by HPLC-DAD analysis. In this case, the LDA was based on the signals of only 4 polymeric membranes. The model allowed to classify correctly 89% and 84%, for the original grouped cases and “leaving one-out” cross-validation procedures, respectively, which is very satisfactory taking in account the higher complexity due to the food matrix effect. Furthermore, only one of the “Gluten-containing” foodstuffs (a bread sample) was misclassified by the model as “Gluten-free”. This misclassification could be tentatively due to an additional difficulty in the gliadins extraction from bread, which is a more processed food matrix. Finally, the work carried out showed that the E-tongue device could be used in practice as a fast and economic preliminary tool to evaluate, in a real time basis, the possible gluten contaminations of “Gluten-free” foodstuffs.
AccessOpen access
Appears in Collections:CEB - Resumos em Livros de Atas / Abstracts in Proceedings

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