Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/16813

TitlePNA-FISH as a new diagnostic method for the determination of clarithromycin resistance of Helicobacter pylori
Author(s)Fernandes, R. M.
Cerqueira, L.
Ferreira, Rui M.
Carneiro, Fátima
Ribeiro, Mário Dinis
Figueiredo, C.
Keevil, C. W.
Azevedo, N. F.
Vieira, M. J.
Issue dateNov-2011
PublisherBioMed Central
JournalBMC Microbiology
Abstract(s)Triple therapy is the gold standard treatment for Helicobacter pylori eradication from the human stomach, but increased resistance to clarithromycin became the main factor of treatment failure. Until now, fastidious culturing methods are generally the method of choice to assess resistance status. In this study, a new genotypic method to detect clarithromycin resistance in clinical samples, based on fluorescent in situ hybridization (FISH) using a set of peptide nucleic acid probes (PNA), is proposed. Results: The set of probes targeting the point mutations responsible for clarithromycin resistance was applied to H. pylori suspensions and showed 100% sensitivity and specificity (95% CI, 79.9-100 and 95% CI, 71.6-100 respectively). This method can also be amenable for application to gastric biopsy samples, as resistance to clarithromycin was also detected when histological slides were tested. Conclusions: The optimized PNA-FISH based diagnostic method to detect H. pylori clarithromycin resistance shown to be a very sensitive and specific method for the detection of clarithromycin resistance in the H. pylori smears and also proved to be a reliable method for the diagnosis of this pathogen in clinical samples and an alternative to existing plating methods.
TypeArticle
URIhttp://hdl.handle.net/1822/16813
DOI10.1186/1471-2180-11-101
ISSN1471-2180
Other identifiers1471-2180
Peer-Reviewedyes
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

Files in This Item:
File Description SizeFormat 
3681.pdf981,85 kBAdobe PDFView/Open

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu ORCID