Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/16792

TitleSeparation of yeasts by addition of flocculent cells of Saccharomyces cerevisiae
Author(s)Mortier, Annelies
Soares, Eduardo V.
KeywordsAdhesion
Aggregation
Cell separation
Co-flocculation
Sedimentation
Yeast
Issue date2007
PublisherSpringer
JournalWorld Journal of Microbiology and Biotechnology
Abstract(s)Separation of yeast cells using a co-flocculation process was investigated. Co-flocculation is a fast process (within few minutes), occurs in a broad pH range (3.0–8.0) and requires a small amount of calcium (0.1 mM). Agitation affects yeast aggregation; however, an agitation between 60 rev/min and 160 rev/min has only a little influence on the co-flocculation process. The ratio flocculent/ non-flocculent cells that induced the settling of 50 and 90% of the cells of S. cerevisiae was 1:7 and 1:1, respectively. Separation of non-flocculent cells can be carried out at any time of the growth cycle. No difference in the efficiency of co-flocculation carried out in buffer (pH 4.0 with 10 mM calcium) or in 48 h-fermented broth was observed. Flocculent cells of Saccharomyces cerevisiae had the ability to sediment non-flocculent cells of S. cerevisiae and Kluyveromyces marxianus, which shows the suitability of the co-flocculation process for separation of different kinds of non-flocculent cells.
TypeArticle
URIhttp://hdl.handle.net/1822/16792
DOI10.1007/s11274-007-9379-6
ISSN1573-0972
Peer-Reviewedyes
AccessRestricted access (UMinho)
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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