Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/16787

Full metadata record
DC FieldValueLanguage
dc.contributor.authorPires, Diana Priscila Penso-
dc.contributor.authorSillankorva, Sanna-
dc.contributor.authorFaustino, A.-
dc.contributor.authorAzeredo, Joana-
dc.date.accessioned2012-02-03T14:43:01Z-
dc.date.available2012-02-03T14:43:01Z-
dc.date.issued2011-
dc.date.submitted2010-
dc.identifier.issn0923-2508por
dc.identifier.urihttp://hdl.handle.net/1822/16787-
dc.description.abstractPseudomonas aeruginosa is a relevant opportunistic pathogen involved in nosocomial infections. that frequently shows low antibiotic susceptibility. One of its virulence factors is associated with the ability to adhere to surfaces and form virulent biofilms. This work describes the isolation and characterization of lytic phages capable of infecting antibiotic-resistant P. aeruginosa strains. In addition, characterization of P. aeruginosa biofilms and the potential of newly isolated phages for planktonic and biofilm control was accessed. According to the results, the isolated phages showed different spectra of activity and efficiency of lysis. Four broad lytic phages were selected for infection of planktonic cells; however, despite their broad range of activity, two of the selected phages failed to efficiently control planktonic cultures. Therefore, only two phages (phiIBB-PAA2 and phiIBB-PAP21), highly capable of causing strong biomass reduction of planktonic cells, were tested against 24 h biofilms using a m.o.i. of 1. Both phages reduced approximately 1-2 log the biofilm population after 2 h of infection and reduction was further enhanced after 6 h of biofilm infection. However, biofilm cells of P. aeruginosa PAO1 acquired resistance to phiIBB-PAP21; consequently, an increase in the number of cells after 24 h of treatment was observed. Conversely, phage phiIB-PAA2 for P. aeruginosa ATCC10145 continued to destroy biofilm cells, even after 24 h of infection. In these biofilms, phages caused a 3 log reduction in the number of viable counts of biofilm cells.por
dc.language.isoengpor
dc.publisherElsevierpor
dc.rightsopenAccesspor
dc.subjectPseudomonas aeruginosapor
dc.subjectBacteriophagespor
dc.subjectBiofilmspor
dc.subjectControlpor
dc.titleUse of newly isolated phages for the control of Pseudomonas aeruginosa PAO1 and ATCC 10145 biofilmspor
dc.typearticlepor
dc.peerreviewedyespor
sdum.publicationstatuspublishedpor
degois.publication.firstPage798por
degois.publication.lastPage806por
degois.publication.issue8por
degois.publication.titleResearch in Microbiologypor
degois.publication.volume162por
dc.identifier.doi10.1016/j.resmic.2011.06.010por
dc.identifier.pmc21782936por
dc.subject.wosScience & Technologypor
sdum.journalResearch in Microbiologypor
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

Files in This Item:
File Description SizeFormat 
3747.pdf494,37 kBAdobe PDFView/Open

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu ORCID