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TitleOil-in-water emulsions characterization by laser granulometry and impact on γ-decalactone production in Yarrowia lipolytica
Author(s)Gomes, Nelma
Waché, Yves
Teixeira, J. A.
Belo, Isabel
KeywordsCastor oil
Droplet size distribution
Methyl ricinoleate
Issue date2011
JournalBiotechnology Letters
Abstract(s)Oil-in-water emulsions composed of methyl ricinoleate (MR) or castor oil (CO) as the organic phase, stabilized by Tween 80, are in the basis of the biotechnological production of gama-decalactone. Y. lipolytica was used due to its ability to grow on hydrophobic substrates and to carry out that biotransformation. The characterization of oil droplets size distribution by laser granulometry was performed under different oil concentrations. The impact of the presence of cells on droplets size was also analyzed, as well as the relevance of washing cells. Furthermore, the granulometric characterization of the emulsions was related with gama-decalactone production for non-washed cells, the smaller droplets disappeared, using both oils, which increased gama-decalactone concentration. This suggests that the access of cells to the substrate occurs by their adhesion around larger oil droplets.
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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