Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/16231

Full metadata record
DC FieldValueLanguage
dc.contributor.authorCaramelo-Nunes, C.-
dc.contributor.authorTente, T.-
dc.contributor.authorAlmeida, P.-
dc.contributor.authorMarcos, João Carlos-
dc.contributor.authorTomaz, C. T.-
dc.date.accessioned2012-01-06T14:14:04Z-
dc.date.available2012-01-06T14:14:04Z-
dc.date.issued2011-
dc.identifier.issn0003-2697por
dc.identifier.urihttp://hdl.handle.net/1822/16231-
dc.description.abstractSmall molecules, like some antibiotics and anticancer agents that bind DNA with high specificity can represent a relevant alternative as ligands in affinity processes for plasmid DNA (pDNA) purification. In the present study, pDNA binding affinities of berberine, berenil, kanamycin and neomycin were evaluated by a competitive displacement assay with ethidium bromide using a fluorimetric titration technique. The binding between pDNA and ethidium bromide was tested in different buffer conditions varying the type and the salt concentration, and was performed both in absence and in presence of the studied compounds. The results showed that the minor groove binder berenil has the higher pDNA binding constant. Chromatographic experiments using a derivatized column with berenil as ligand, showed a total retention of pDNA using 1.3 M ammonium sulphate in eluent buffer. A selective separation of supercoiled and open circular isoforms was achieved by further decreasing salt concentration to 0.6 M and then to 0 M. These results suggest a promising application of berenil as ligand for specific pDNA supercoiled (sc) isoform purification by pseudo-affinity chromatography.por
dc.description.sponsorshipC. Caramelo-Nunes acknowledges a fellowship (SFRH/BD/64918/2009) from the Portuguese Foundation for Science and Technology (FCT).por
dc.language.isoengpor
dc.publisherElsevierpor
dc.rightsopenAccesspor
dc.subjectSmall DNA ligandspor
dc.subjectBerenilpor
dc.subjectMinor groove binderpor
dc.subjectIntercalatorpor
dc.subjectPseudo-affinity chromatographypor
dc.titleSpecific berenil–DNA interactions : an approach for separation of plasmid isoforms by pseudo–affinity chromatographypor
dc.typearticlepor
dc.peerreviewedyespor
sdum.publicationstatuspublishedpor
oaire.citationStartPage153por
oaire.citationEndPage158por
oaire.citationIssue2por
oaire.citationTitleAnalytical Biochemistrypor
oaire.citationVolume412por
dc.identifier.doi10.1016/j.ab.2011.01.033-
dc.identifier.pmid21284930por
dc.subject.wosScience & Technologypor
sdum.journalAnalytical Biochemistrypor
Appears in Collections:CDQuim - Artigos (Papers)

Files in This Item:
File Description SizeFormat 
ArtigoInPress.pdfDocumento Principal429,23 kBAdobe PDFView/Open

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu ORCID