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TitleStudies on the properties of Celluclast/Eudragit L-100 conjugate
Author(s)Dourado, Fernando
Bastos, Maria de Lurdes
Mota, M.
Gama, F. M.
Eudragit L-100
Enzyme immobilization
Issue date2002
JournalJournal of Biotechnology
Citation“Journal of Biotechnology”. ISSN 0168-1656. 99:2 (2002) 121-131.
Series/Report no.2002;08
Abstract(s)A cellulase from Trichoderma reesei was immobilized on Eudragit L-100, a reversibly soluble polymer depending on the pH of the medium. The solubility of the modified cellulase was studied at different pH values. By changing the pH, the adsorption equilibrium of the derivatized proteins is switched towards the liquid phase, thus making recycling possible. This method allows for improved stability, without major loss of specific activity. The adsorption of cellulase on Eudragit lowers the enthalpy of denaturation, but affects only slightly the denaturation temperature. The use of carbodiimide was ineffective on linking the enzymes covalently to the polymer, since the immobilization process was found to be only mediated by non-covalent forces.
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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