Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/14403

TitleThermoresponsive poly(N-isopropylacrylamide)-g-methylcellulose hydrogel as a three-dimensional extracellular matrix for cartilage-engineered applications
Author(s)Sá-Lima, Helena
Tuzlakoglu, K.
Mano, J. F.
Reis, R. L.
KeywordsInjectable
Hydrogel
Hydrogel, thermoresponsive, cell encacartilage tissue engineering
Thermoresponsive
cell encapsulation
cartilage tissue engineering
Issue date15-Sep-2011
PublisherWiley
JournalJournal of Biomedical Materials Research Part A
Abstract(s)Recent advances in tissue engineering and regenerative medicine fields can offer alternative solutions to the existing techniques for cartilage repair. In this context, a variety of materials has been proposed, and the injectable hydrogels are among the most promising alternatives. The aim of this work is to explore the ability of poly(N-isopropylacrylamide)- g-methylcellulose (PNIPAAm-g-MC) thermoreversible hydrogel as a three-dimensional support for cell encapsulation toward the regeneration of articular cartilage through a tissue engineering approach. The PNIPAAm-g-MC copolymer was effectively obtained using ammonium-persulfate and N,N,N0,N0-tetramethylethylenediamine as initiator as confirmed by Fourier transform infrared spectroscopy and 1H NMR results. The copolymer showed to be temperature responsive, becoming a gel at temperatures above its lower critical solution temperature ( 32 C) while turning into a liquid below it. Results obtained from the MTS test showed that extracts of the hydrogel were clearly noncytotoxic to L929 fibroblast cells. ATDC5 cells, a murine chondrogenic cell line, were used as the in vitro model for this study; they were encapsulated at high cell density within the hydrogel and cultured for up to 28 days. PNIPAAm-g-MC did not affect the cell viability and proliferation, as indicated by both MTS and DNA assays. The results also revealed an increase in synthesis of glycosoaminoglycans within culture time measured by the dimethylmethylene blue quantification assay. These results suggest the viability of using PNIPAAm-g-MC thermoresponsive hydrogel as a three-dimensional scaffold for cartilage tissue engineering using minimal-invasive strategies.
Typearticle
URIhttp://hdl.handle.net/1822/14403
DOI10.1002/jbm.a.33140
ISSN1549-3296
Peer-Reviewedyes
AccessrestrictedAccess
Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals

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