Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/14269

TitleAssessment of bone formation markers under controlled environmental conditions and their correlations with serum minerals in the adult sheep as a model for orthopaedic research
Author(s)Dias, Isabel R.
Viegas, Carlos A.
Azevedo, Jorge T.
Costa, E. M.
Lourenço, P.
Rodrigues, A.
Cabrita, A. S.
KeywordsTotal and bone-specific alkaline phosphatase
Osteocalcin
Minerals
Serum
Issue date2008
JournalLaboratory Animals
Abstract(s)Eighteen healthy skeletally mature (3 years old) ewes, with an average weight of 45 kg, of the Portuguese Churra da Terra Quente breed were used to evaluate the normal values of total and bone-specific isoform of alkaline phosphatase serum activities (ALP and BALP, respectively) and serum osteocalcin (OC) and their correlation with the serum minerals – calcium (Ca), phosphorus (P), magnesium (Mg) and ionized calcium (Ca2þ). The sheep were maintained under controlled environmental conditions (constant diurnal photoperiod cycle and identical husbandry and feeding) for six weeks before the collection of the blood samples. The measurement of the total ALP and serum minerals was performed with automated biochemistry analysers using the BioMe´rieuxw kits, the serum electrolyte Ca2þ Diametrics Medical, Incw specific cassettes and the BALP and OC METRATM kits from QUIDELw Corporation. The mean+standard deviation values obtained were: total ALP 90.17+ 85.72 U/L, BALP 15.0+5.44 U/L, ratio BALP/ total ALP 29.28+24.22, OC 13.02+ 1.87 ng/mL, Ca 2.57+0.37 mmol/L, P 2.13+0.42 mmol/L, Mg 1.04+0.13 mmol/L, Ca2þ 1.29+0.04 mmol/L. Significant correlations were observed between the total ALP and Ca (r ¼ 0.5939; P ¼ 0.05) and OC and Ca (r ¼ 0.5706; P ¼ 0.05). Reference to the serum values of bone turnover parameters in sheep could be of great value in research and could provide complementary non-invasive information on the bone healing process, particularly with regard to obtaining an early prognosis of fracture healing.
TypeArticle
URIhttp://hdl.handle.net/1822/14269
Peer-Reviewedyes
AccessOpen access
Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals

Files in This Item:
File Description SizeFormat 
file.pdf90,02 kBAdobe PDFView/Open

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu ORCID