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TitleExpression, purification and osteogenic bioactivity of recombinant human BMP-4, -9, -10, -11 and -14
Author(s)Bessa, Paulo C.
Cerqueira, M. T.
Rada, Tommaso
Gomes, Manuela E.
Neves, N. M.
Nobre, Alexandra
Reis, R. L.
Casal, Margarida
KeywordsBone morphogenetic protein-4
Bone morphogenetic protem-9
Bone morphogenetic protein-10
Growth differentiation factor-11
Growth differentiation factor-5
Bone morphogenetic protein-9
Issue date2009
JournalProtein Expression and Purification
Abstract(s)Bone morphogenetic proteins (BMPs) are cytokines from the TGF-b superfamily, with important roles during embryonic development and in the induction of bone and cartilage tissue differentiation in the adult body. In this contribution, we report the expression of recombinant human BMP-4, BMP-9, BMP-10, BMP-11 (or growth differentiation factor- 11, GDF-11) and BMP-14 (GDF-5), using Escherichia coli pET-25b vector. BMPs were overexpressed, purified by affinity his-tag chromatography and shown to induce the expression of early markers of bone differentiation (e.g. smad-1, smad-5, runx2/cbfa1, dlx5, osterix, osteopontin, bone sialoprotein and alkaline phosphatase) in C2C12 cells and in human adipose stem cells. The described approach is a promising method for producing large amounts of different recombinant BMPs that show potential for novel biomedical applications.
AccessOpen access
Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals
DBio - Artigos/Papers

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