Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/14106
Registo completo
Campo DC | Valor | Idioma |
---|---|---|
dc.contributor.author | Benesch, Johan | - |
dc.contributor.author | Svedhem, S. | - |
dc.contributor.author | Svensson, S. C. | - |
dc.contributor.author | Valiokas, R. | - |
dc.contributor.author | Liedberg, B. | - |
dc.contributor.author | Tengvall, P. | - |
dc.date.accessioned | 2011-11-02T15:02:00Z | - |
dc.date.available | 2011-11-02T15:02:00Z | - |
dc.date.issued | 2001 | - |
dc.identifier.issn | 0920-5063 | por |
dc.identifier.uri | https://hdl.handle.net/1822/14106 | - |
dc.description.abstract | Low protein adsorption is believed advantageous for blood-contacting materials and ethylene glycols (EG)-based polymeric compounds are often attached to surfaces for this purpose. In the present study, the adsorption of brinogen, serum, and plasma were studied by ellipsometry on a series of well-de ned oligo(EG) terminated alkane-thiols self-assembled on gold. The layers were prepared with compounds of the general structure HS-(CH2)15-CONH-EGn, where n D 2, 4, and 6. Methoxy-terminated tri(EG) undecanethiol and hydroxyl-terminated hexadecanethiol self-assembled monolayers (SAMs) were used as references. The results clearly demonstrate that the adsorption depends on the experimental conditions with small amounts of brinogen adsorbing from a single protein solution, but larger amounts of proteins from serum and plasma. The adsorption of brinogen and blood plasma decreased with an increasing number of EG repeats and was temperature-dependent. Signi cantly less serum adsorbed to methoxy tri(EG) than to hexa(EG) and more proteins remained on the latter surface after incubation in a sodiumdodecyl sulfate (SDS) solution, indicating a looser protein binding to the methoxy-terminated surface. All surfaces adsorbed complement factor 3 (C3) from serum and plasma, although no surfacemediated complement activationwas observed. The present study points to the importance of a careful choice of the proteinmodel system before general statements regardingthe protein repellantproperties of potential surfaces can be made. | por |
dc.language.iso | eng | por |
dc.rights | openAccess | por |
dc.subject | Self-assembled monolayers | por |
dc.subject | Oligo(ethylene glycol) | por |
dc.subject | Protein adsorption | por |
dc.subject | Fibrinogen | por |
dc.subject | Heparinized plasma | por |
dc.subject | Serum | por |
dc.subject | Complement | por |
dc.title | Protein adsorption to oligo (Ethylene Glycol) self assembled monolayers with amide linkage experiments with fibrinogen, heparinised plasma and serum | por |
dc.type | article | por |
dc.peerreviewed | yes | por |
sdum.publicationstatus | published | por |
oaire.citationStartPage | 581 | por |
oaire.citationEndPage | 597 | por |
oaire.citationIssue | 6 | por |
oaire.citationTitle | Journal of Biomaterials Science Polymer Edition | por |
oaire.citationVolume | 12 | por |
sdum.journal | Journal of Biomaterials Science. Polymer Edition | por |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |