Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/14106
Título: | Protein adsorption to oligo (Ethylene Glycol) self assembled monolayers with amide linkage experiments with fibrinogen, heparinised plasma and serum |
Autor(es): | Benesch, Johan Svedhem, S. Svensson, S. C. Valiokas, R. Liedberg, B. Tengvall, P. |
Palavras-chave: | Self-assembled monolayers Oligo(ethylene glycol) Protein adsorption Fibrinogen Heparinized plasma Serum Complement |
Data: | 2001 |
Revista: | Journal of Biomaterials Science. Polymer Edition |
Resumo(s): | Low protein adsorption is believed advantageous for blood-contacting materials and ethylene glycols (EG)-based polymeric compounds are often attached to surfaces for this purpose. In the present study, the adsorption of brinogen, serum, and plasma were studied by ellipsometry on a series of well-de ned oligo(EG) terminated alkane-thiols self-assembled on gold. The layers were prepared with compounds of the general structure HS-(CH2)15-CONH-EGn, where n D 2, 4, and 6. Methoxy-terminated tri(EG) undecanethiol and hydroxyl-terminated hexadecanethiol self-assembled monolayers (SAMs) were used as references. The results clearly demonstrate that the adsorption depends on the experimental conditions with small amounts of brinogen adsorbing from a single protein solution, but larger amounts of proteins from serum and plasma. The adsorption of brinogen and blood plasma decreased with an increasing number of EG repeats and was temperature-dependent. Signi cantly less serum adsorbed to methoxy tri(EG) than to hexa(EG) and more proteins remained on the latter surface after incubation in a sodiumdodecyl sulfate (SDS) solution, indicating a looser protein binding to the methoxy-terminated surface. All surfaces adsorbed complement factor 3 (C3) from serum and plasma, although no surfacemediated complement activationwas observed. The present study points to the importance of a careful choice of the proteinmodel system before general statements regardingthe protein repellantproperties of potential surfaces can be made. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/14106 |
ISSN: | 0920-5063 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |