Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/14042
Registo completo
Campo DC | Valor | Idioma |
---|---|---|
dc.contributor.author | Bessa, Paulo C. | - |
dc.contributor.author | Pedro, A. J. | - |
dc.contributor.author | Klosch, B. | - |
dc.contributor.author | Nobre, Alexandra | - |
dc.contributor.author | Griensven, Martijn van | - |
dc.contributor.author | Reis, R. L. | - |
dc.contributor.author | Casal, Margarida | - |
dc.date.accessioned | 2011-10-26T14:48:57Z | - |
dc.date.available | 2011-10-26T14:48:57Z | - |
dc.date.issued | 2008 | - |
dc.identifier.issn | 0141-5492 | por |
dc.identifier.uri | https://hdl.handle.net/1822/14042 | - |
dc.description.abstract | Bioactive recombinant human bone morphogenetic protein-2 (rhBMP-2) was obtained using Escherichia coli pET-25b expression system: 55 mg purified rhBMP-2 were achieved per g cell dry wt, with up to 95% purity. In murine C2C12 cell line, rhBMP-2 induced an increase in the transcription of Smads and of osteogenic markers Runx2/Cbfa1 and Osterix, measured by semi-quantitative RT-PCR. Bioassays performed in human fat-derived stem cells showed an increased activity of the early osteogenic marker, alkaline phosphatase, and the absence of cytotoxicity. | por |
dc.language.iso | eng | por |
dc.publisher | Springer | por |
dc.rights | openAccess | por |
dc.subject | Bone morphogenetic protein-2 | por |
dc.subject | Recombinant protein purification | por |
dc.subject | Stem cells | por |
dc.subject | Tissue engineering | por |
dc.title | Osteoinduction in human fat derived stem cells by recombinant human bone morphogenetic protein-2 produced in Escherichia coli | por |
dc.type | article | por |
dc.peerreviewed | yes | por |
sdum.publicationstatus | published | por |
oaire.citationStartPage | 15 | por |
oaire.citationEndPage | 21 | por |
oaire.citationIssue | 1 | - |
oaire.citationTitle | Biotechnology Letters | por |
oaire.citationVolume | 30 | - |
dc.identifier.doi | 10.1007/s10529-007-9501-8 | por |
dc.identifier.pmid | 17673947 | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Biotechnology Letters | por |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals DBio - Artigos/Papers |