Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/14037

Full metadata record
DC FieldValueLanguage
dc.contributor.authorSantos, T. C.-
dc.contributor.authorMarques, A. P.-
dc.contributor.authorSilva, Simone Santos-
dc.contributor.authorOliveira, Joaquim M.-
dc.contributor.authorMano, J. F.-
dc.contributor.authorCastro, António G.-
dc.contributor.authorGriensven, Martijn van-
dc.contributor.authorReis, R. L.-
dc.date.accessioned2011-10-26T14:20:18Z-
dc.date.available2011-10-26T14:20:18Z-
dc.date.issued2010-
dc.identifier.issn2152-4947por
dc.identifier.issn2152-4955por
dc.identifier.urihttp://hdl.handle.net/1822/14037-
dc.description.abstractSoybean protein has been proposed for distinct applications within nutritional, pharmaceutical, and cosmetic industries among others. More recently, soy-based biomaterials have also demonstrated promising properties for biomedical applications.However, althoughmany reports within other fields exist, the inflammatory/immunogenic potential of those materials is still poorly understood and therefore can hardly be controlled. On the contrary, chitosan (Cht) has been well explored in the biomedical field, either by itself or combined with synthetic or other natural-based polymers. Therefore, the combination of chitosan with soybean protein is foreseen as a suitable approach to control the biological behavior of soy-based biomaterials. Under this context this work was designed to try to understand the influence of chitosan in the host response elicited by soy-based biomaterials. Soybean protein isolate powder (SI-P) and Cht powder (Cht-P) were injected as suspension into the intraperitoneal cavity of rats. SI-P induced the recruitment of higher numbers of leukocytes compared to the Cht-P during the entire observation period. In this sense, SI-P elicited a considerable reaction from the host comparing to the Cht-P, which elicited leukocyte recruitment similar to the negative control.After subcutaneous implantation of the soybean and denatured membranes, (SI-M and dSI-M) a severe host inflammatory reaction was observed. Conversely, Cht/soy-based membranes (Cht/soy-based membranes) showed the induction of a normal host response after subcutaneous implantation in rats, which allowed concluding that the addition of chitosan to the soy-based membranes improved their in vivo performance. Thus, the presented results assert the improvement of the host response, considering inflammatory cells recruitment, and overall inflammatory reaction,when chitosan is combined to soybean. Together with previous results that reported their promising physicochemical characteristics and their inability to activate human polymorphonuclear neutrophils in vitro, the herein presented conclusions reinforce the usefulness of theCht/ soy-based membranes and justify the pursue for a specific application within the biomedical field.por
dc.description.sponsorshipThe author Tircia C. Santos acknowledges the Marie Curie European Program for a short-term scholarship in the Alea Jacta EST project (MEST-CT-2004-008104). This work was developed under the scope of the European Network of Excellence EXPERTISSUES (NMP3-CT-2004-5000283).por
dc.language.isoengpor
dc.publisherMary Ann Liebertpor
dc.rightsopenAccesspor
dc.titleChitosan improves the biological performance of soy-based biomaterialspor
dc.typearticlepor
dc.peerreviewedyespor
sdum.publicationstatuspublishedpor
oaire.citationStartPage2883por
oaire.citationEndPage2890por
oaire.citationIssue9por
oaire.citationTitleTissue Engineeringpor
oaire.citationVolume16por
dc.identifier.doi10.1089/ten.tea.2010.0114por
dc.identifier.pmid20486796por
dc.subject.wosScience & Technologypor
sdum.journalTissue Engineeringpor
Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals

Files in This Item:
File Description SizeFormat 
file.pdf383,51 kBAdobe PDFView/Open

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu ORCID