Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/13847
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Campo DC | Valor | Idioma |
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dc.contributor.author | Silva, Carla Manuela Pereira Marinho | - |
dc.contributor.author | Silva, Carla J. S. M. | - |
dc.contributor.author | Zille, Andrea | - |
dc.contributor.author | Gübitz, Georg M. | - |
dc.contributor.author | Paulo, Artur Cavaco | - |
dc.date.accessioned | 2011-10-12T08:40:23Z | - |
dc.date.available | 2011-10-12T08:40:23Z | - |
dc.date.issued | 2007-11 | - |
dc.date.submitted | 2007 | - |
dc.identifier.issn | 0141-0229 | por |
dc.identifier.uri | https://hdl.handle.net/1822/13847 | - |
dc.description.abstract | Polyamide matrices, such as membranes, gels and non-wovens, have been applied as supports for enzyme immobilization, although in literature the enzyme immobilization on woven nylon matrices is rarely reported. In this work, a protocol for a Trametes hirsuta laccase immobilization using woven polyamide 6,6 (nylon) was developed. A 24 full factorial design was used to study the influence of pH, spacer (1,6-hexanediamine), enzyme and crosslinker concentration on the efficiency of immobilization. The factors enzyme dosage and spacer seem to have played a critical role in the immobilization of laccase onto nylon support. Under optimized working conditions (29 U mL−1 of laccase, 10% of glutaraldehyde, pH = 5.5, with the presence of the spacer), the half-life time attained was about 78 h (18% higher than that of free enzyme), the protein retention was 30% and the immobilization yield was 2%. The immobilized laccase has potential for application in the continuous decolourization of textile effluents, where it can be applied into a membrane reactor. | por |
dc.language.iso | eng | por |
dc.publisher | Elsevier | por |
dc.rights | openAccess | por |
dc.subject | Polyamide | por |
dc.subject | Nylon | por |
dc.subject | Immobilization | por |
dc.subject | Crosslinking | por |
dc.subject | Glutaraldehyde | por |
dc.subject | Laccase | por |
dc.title | Laccase immobilization on enzymatically functionalized polyamide 6,6 fibres | por |
dc.type | article | por |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | http://www.sciencedirect.com/science/article/pii/S0141022907002463 | por |
sdum.publicationstatus | published | por |
oaire.citationStartPage | 867 | por |
oaire.citationEndPage | 875 | por |
oaire.citationIssue | 6-7 | por |
oaire.citationTitle | Enzyme and Microbial Technology | por |
oaire.citationVolume | 41 | por |
dc.identifier.doi | 10.1016/j.enzmictec.2007.07.010 | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Enzyme and Microbial Technology | por |
Aparece nas coleções: | DET/2C2T - Artigos em revistas internacionais com arbitragem científica |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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Laccase Immobilization on Enzymatically Functionalized Polyamide 6,6 Fibres.pdf | Laccase immobilization on enzymatically functionalized polyamide 6,6 fibres | 543,39 kB | Adobe PDF | Ver/Abrir |