Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/13597

TitleNanoliposomes for encapsulation and delivery of the potential antitumoral methyl 6-methoxy-3-(4-methoxyphenyl)-1H-indole-2-carboxylate
Author(s)Abreu, Ana S.
Castanheira, Elisabete M. S.
Queiroz, Maria João R. P.
Ferreira, Paula M. T.
Silva, Luís A. Vale
Pinto, Eugénia
KeywordsNanoliposomes
Antitumoral drugs
Fluorescence
Dialysis
Issue dateAug-2011
PublisherSpringerOpen
JournalNanoscale Research Letters
Abstract(s)A potential antitumoral fluorescent indole derivative, methyl 6-methoxy-3-(4-methoxyphenyl)-1H-indole-2-carboxylate, was evaluated for the in vitro cell growth inhibition on three human tumor cell lines, MCF-7 (breast adenocarcinoma), A375-C5 (melanoma), and NCI-H460 (non-small cell lung cancer), after a continuous exposure of 48 h, exhibiting very low GI50 values for all the cell lines tested (0.25 to 0.33 µM). This compound was encapsulated in different nanosized liposome formulations, containing egg lecithin (Egg-PC), dipalmitoyl phosphatidylcholine (DPPC), dipalmitoyl phosphatidylglycerol (DPPG), DSPC, cholesterol, dihexadecyl phosphate, and DSPE-PEG. Dynamic light scattering measurements showed that nanoliposomes with the encapsulated compound are generally monodisperse and with hydrodynamic diameters lower than 120 nm, good stability and zeta potential values lower than −18 mV. Dialysis experiments allowed to monitor compound diffusion through the lipid membrane, from DPPC/DPPG donor liposomes to NBD-labelled lipid/DPPC/DPPG acceptor liposomes.
TypeArticle
URIhttp://hdl.handle.net/1822/13597
DOI10.1186/1556-276X-6-482
ISSN1556-276X
Publisher versionhttp://www.nanoscalereslett.com
Peer-Reviewedyes
AccessOpen access
Appears in Collections:CDF - FAMO - Artigos/Papers (with refereeing)
CDQuim - Artigos (Papers)

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