Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/13134

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dc.contributor.authorAraújo, Rita-
dc.contributor.authorSilva, Carla Manuela Pereira Marinho da-
dc.contributor.authorO'Neill, Jaime Alexandre Antunes-
dc.contributor.authorMicaelo, N. M.-
dc.contributor.authorGübitz, Georg M.-
dc.contributor.authorSoares, Cláudio M.-
dc.contributor.authorCasal, Margarida-
dc.contributor.authorPaulo, Artur Cavaco-
dc.date.accessioned2011-07-27T16:01:59Z-
dc.date.available2011-07-27T16:01:59Z-
dc.date.issued2007-03-
dc.identifier.issn0168-1656por
dc.identifier.urihttp://hdl.handle.net/1822/13134-
dc.description.abstractCutinase from Fusarium solani pisi was genetically modified near the active site, by site-directed mutagenesis, to enhance its activity towards polyethylene terephthalate (PET) and polyamide 6,6 (PA 6,6) fibers. The mutations L81A, N84A, L182A, V184A and L189A were done to enlarge the active site in order to better fit a larger polymer chain. Modeling studies have shown enhanced free energy stabilization of model substrate tetrahedral intermediate (TI) bound at the enzyme active site for all mutants, for both model polymers. L81A and L182A showed an activity increase of four- and five-fold, respectively, when compared with the wild type, for PET fibers. L182A showed the one- and two-fold higher ability to biodegrade aliphatic polyamide substrates. Further studies in aliphatic polyesters seem to indicate that cutinase has higher ability to recognize aliphatic substrates.por
dc.description.sponsorshipFundação para a Ciência e a Tecnologia (FCT) - SFRH/BD/22490/2005, SFRH/BD/22149/2005por
dc.description.sponsorshipEuropean Community - Biosyntex Project, no. G5RD-CT-2000-30110 “Competitive and Sustainable Growth”por
dc.language.isoengpor
dc.publisherElsevierpor
dc.rightsopenAccesspor
dc.subjectCutinasepor
dc.subjectPolyesterpor
dc.subjectPolyamide 6,6por
dc.subjectSite-directed mutagenesispor
dc.subjectBiocatalysispor
dc.titleTailoring cutinase activity towards polyethylene terephthalate and polyamide 6,6 fiberspor
dc.typearticlepor
dc.peerreviewedyespor
dc.relation.publisherversionhttp://www.sciencedirect.com/por
sdum.publicationstatuspublishedpor
oaire.citationStartPage849por
oaire.citationEndPage857por
oaire.citationIssue4por
oaire.citationTitleJournal of Biotechnologypor
oaire.citationVolume128por
dc.identifier.doi10.1016/j.jbiotec.2006.12.028por
dc.identifier.pmid17306400por
dc.subject.wosScience & Technologypor
sdum.journalJournal of Biotechnologypor
Appears in Collections:DET/2C2T - Artigos em revistas internacionais com arbitragem científica

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