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TitleMaximization of fructose esters synthesis by response surface methodology
Author(s)Neta, Nair S.
Peres, A. M.
Teixeira, J. A.
Rodrigues, L. R.
KeywordsCandida antartica B
Fructose esters
Response surface methodology
Issue date2011
PublisherEuropean Federation of Biotechnology (EFB)
JournalNew Biotechnology
Abstract(s)Enzymatic synthesis of fructose fatty acide sterwasper formed inorganic solventmedia, using apurified lipase from Candida antartica B immobilizedinacrylicresin. Response sur face methodology with a central composit erotatable design based on five levels was implemented to optimize three experimental operating conditions (temperature, agitation and reaction time). A statistical significant cubic model was established. Temperature andre action time were found to bethe most significant parameters. The optimum operational conditions for maximizing the synthesis of fructos e esters were 57.18C, 100rpm and 37.8h. The model was validated in the identified optimal condition stocheck its adequacy and accuracy, and an experimental esterification percentage of 88.4% ( 0.3%) was obtained. These results showed that animprovement of the enzymatic synthesis of fructose esters was obtained under the optimized conditions
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AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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