Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/12946

TitleNew model substrates for enzymes hydrolysing polyethyleneterephthalate and polyamide fibres
Author(s)Heumann, Sonja
Eberel, Anita
Pobeheim, Herbert
Liebminger, Stefan
Fischer-Colbrie, Gudrun
Almansa, Eva
Paulo, Artur Cavaco
Gübitz, Georg M.
KeywordsPolyamide
Model substrate
Polyethyleneterphthalate
Enzyme
Issue date30-Nov-2006
PublisherElsevier
JournalJournal Biochemical and Biophysical Methods
Abstract(s)Recently the potential of enzymes for surface hydrophilisation and/or functionalisation of polyethyleneterephthalate (PET) and polyamide (PA) has been discovered. However, there was no correlation between enzyme class/activity (e.g. esterase, lipase, cutinase) and surface hydrolysis of these polymers and consequently no simple assay to estimate this capability. Enzymes active on the model substrates bis (benzoyloxyethyl) terephthalate and adipic acid bishexyl-amide, were also capable of increasing the hydrophilicity of PET and PA. When dosed at the identical activity on 4-nitrophenyl butyrate, only enzymes from Thermobifida fusca, Aspergillus sp., Beauveria sp. and commercial enzymes (TEXAZYME PES sp5 and Lipase PS) increased the hydrophilicity of PET fibres while other esterases and lipases did not show any effect. Activity on PET correlated with the activity on the model substrate. Hydrophilicity of fibres was greatly improved based on increases in rising height of up to 4.3 cm and the relative decrease of water absorption time between control and sample of the water was up to 76%. Similarly, enzymes increasing the hydrophilicity of PA fibres such as from Nocardia sp., Beauveria sp. and F. solani hydrolysed the model substrate; however, there was no common enzyme activity (e.g. protease, esterase, amidase) which could be attributed to all these enzymes.
TypeArticle
URIhttp://hdl.handle.net/1822/12946
DOI10.1016/j.jbbm.2006.02.005
ISSN0165-022X
Publisher versionhttp://www.sciencedirect.com/science/article/pii/S0165022X06000340
Peer-Reviewedyes
AccessOpen access
Appears in Collections:DET/2C2T - Artigos em revistas internacionais com arbitragem científica

Files in This Item:
File Description SizeFormat 
NEW MODEL SUBSTRATES FOR ENZYMES HYDROLYZING POLYETHYLENETEREPHTHALATE AND POLYAMIDE FIBRES.pdfNew model substrates for enzymes hydrolysing polyethyleneterephthalate and polyamide fibres153,57 kBAdobe PDFView/Open

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu ORCID