Utilize este identificador para referenciar este registo: http://hdl.handle.net/1822/11212

TítuloCandida clinical species identification : molecular and biochemical methods
Autor(es)Costa, A. R.
Silva, F.
Henriques, Mariana
Azeredo, Joana
Oliveira, Rosário
Faustino, A.
Palavras-chaveCandida species
Discriminating potential
PCR
Vitek 2 YST
Auxacolor
ID32C
Data2010
EditoraSpringer Verlag
RevistaAnnals of Microbiology
Citação"Annals of Microbiology". ISSN 1590-4261. 60:1 (2010) 105-112.
Resumo(s)In the last decade, the number and diversity of nosocomial Candida infections has increased significantly, resulting in an emergent need for rapid and accurate methods for Candida identification. Therefore, the aim of this study was to evaluate the performance of three biochemical systems (Auxacolor, ID32C, and Vitek 2 YST) for the identification of Candida species, comparing them with molecular identification (polymerase chain reaction and gel agarose electrophoresis). These methods were used to assess Candida spp. (229 clinical isolates) prevalence and distribution among clinical specimens. The biochemical methods with higher percentages of correct identification were Vitek 2 YST (79.6%) and Auxacolor (78.6%). However, overall the biochemical methods assayed differed from the molecular identification. Thus, due to their rapid and precise identification, molecular methods are promising techniques for Candida species identification in clinical laboratories. Candida albicans and Non Candida albicans Candida species had a similar prevalence (50.4 and 49.6%, respectively), corroborating the epidemiological shift observed for these pathogens in the recent years.
Tipoarticle
URIhttp://hdl.handle.net/1822/11212
DOI10.1007/s13213-009-0007-6
ISSN1590-4261
Arbitragem científicayes
AcessoopenAccess
Aparece nas coleções:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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