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TitleBiological activity of heterologous murine interleukin-10 and preliminary studies on the use of a dextrin nanogel as a delivery system
Author(s)Carvalho, Vera
Castanheira, Pedro
Faria, Tiago Q.
Gonçalves, Catarina
Madureira, Pedro
Faro, Carlos
Domingues, Lucília
Brito, Rui M. M.
Vilanova, Manuel
Gama, F. M.
KeywordsProtein delivery system
Anti-inflammatory cytokine
Endotoxin-activated macrophages
Dextrin nanogel
Issue date2010
JournalInternational Journal of Pharmaceutics
Citation"International Journal of Pharmaceutics". ISSN 0378-5173. 400:1-2 (2010) 234-242.
Abstract(s)Interleukin-10 (IL-10) is an anti-inflammatory cytokine, which active form is a non-covalent homodimer with two intramolecular disulphide bonds essential for its biological activity. A mutated form of murine IL-10 was successfully expressed in E. coli, recovered and purified from inclusion bodies. Its ability to reduce tumor necrosis factor synthesis and down-regulate class II major histocompatibility complex molecules expression on endotoxin-stimulated bone marrow-derived macrophages was confirmed, and shown to be similar to that of a commercially available IL-10. Given the potential of IL-10 for application in various medical conditions, it is essential to develop systems that can effectively deliver the protein. In this work it is shown that a dextrin nanogel effectively incorporate IL-10, stabilize, and enable the slow release of biologically active IL-10 over time. Altogether, these results demonstrate the suitability of dextrin nanogel to be used as a system for the controlled release of IL-10.
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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