Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/1086

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dc.contributor.authorLemos, M. A.-
dc.contributor.authorTeixeira, J. A.-
dc.contributor.authorDomingues, M. R. M.-
dc.contributor.authorMota, M.-
dc.contributor.authorGama, F. M.-
dc.date.accessioned2005-04-15T14:13:53Z-
dc.date.available2005-04-15T14:13:53Z-
dc.date.issued2003-
dc.identifier.citation"Enzyme and microbial technology". ISSN 0141-0229. 32:1 (2003) 35-40.eng
dc.identifier.issn0141-0229por
dc.identifier.urihttp://hdl.handle.net/1822/1086-
dc.description.abstractThe effect of isolated cellulose binding domains (CBDs) on the hydrolysis of filter paper and microcrystalline cellulose by both cellobiohydrolase I and endoglucanase, was studied. CBDs were obtained by proteolysis from cellulases using a scaled-up variant of our previous method. Experiments were performed for different enzyme/substrate ratios in both the absence and presence of CBDs. Hydrolysis of filter paper by intact cellobiohydrolase I in the presence of additional CBDs was found to have a synergistic effect, leading to an increase of the sugar production of up to 30%. The effect was less pronounced using microcrystalline cellulose, where an increase up to 16% was observed. Similar trends were found during the hydrolysis of both substrates by endoglucanase.eng
dc.description.sponsorshipFundação para a Ciência e a Tecnologia (FCT) – PRAXIS XXI.por
dc.language.isoeng-
dc.publisherElseviereng
dc.relation.ispartofseries2003;19-
dc.rightsopenAccesseng
dc.subjectCellulose binding domainseng
dc.subjectSynergismeng
dc.subjectCellulaseseng
dc.titleThe enhancement of the cellulolytic activity of cellobiohydrolase I and endoglucanase by the addition of cellulose binding domains derived from trichoderma reeseieng
dc.typearticle-
dc.peerreviewedyeseng
oaire.citationStartPage35por
oaire.citationEndPage40por
oaire.citationIssue1por
oaire.citationVolume32por
dc.identifier.doi10.1016/S0141-0229(02)00235-1por
dc.subject.wosScience & Technologypor
sdum.journalEnzyme and Microbial Technologypor
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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