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TitleA comparison between bright field and phase-contrast image analysis techniques in activated sludge morphological characterization
Author(s)Mesquita, D. P.
Dias, Oscar
Amaral, A. L.
Ferreira, E. C.
KeywordsActivated sludge
Image analysis
Bright field
Phase contrast
Issue dateApr-2010
PublisherMicroscopy Society of America
JournalMicroscopy and Microanalysis
Citation"Microscopy and Microanalysis". ISSN 1431-9276. 16:2 (2010) 166-174.
Abstract(s)Different approaches using microscopy image analysis procedures were employed for characterization of activated sludge systems. The approaches varied mainly on the type of visualization and acquisition method used for collection of data. In this context, this study focused on the comparison of the two most common acquisition methods: bright field and phase-contrast microscopy. Images were acquired from seven different wastewater treatment plants for a combined period of two years. Advantages and disadvantages of each acquisition technique and the results are discussed. Bright field microscopy proved to be more simple and inexpensive and provided the best overall results.
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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