Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/1051

TitleCellulases for oligosaccharide synthesis : a preliminary study
Author(s)Gama, F. M.
Mota, M.
KeywordsCellulases
Oligosaccharide
Enzymes
Issue date1998
PublisherElsevier
JournalCarbohydrate Polymers
Citation“Carbohydrate polymers”. ISSN 0144-8617. 37 (1998) 279–281.
Series/Report no.1998;13
Abstract(s)Crude cellulases, cellobiohydrolase I and endoglucanase I from Trichoderma reesei, were used for preliminary analysis of the potential of cellulases for oligosaccharide synthesis. The reaction products were analysed by ionic chromatography. Synthesis activity could be detected in concentrated aqueous solutions of glucose, cellobiose, maltose and raffinose. CBH I and EG I showed similar activity, with the exception of the case where cellobiose was the substrate. In this case, CBH I originated more ligosaccharides and in higher yields. For the best case among the studied experimental conditions (enzyme: CBH I; substrate: cellubiose; temperature: 4ºC), yields in the order of 40% oligosaccharides were obtained. The effect of the temperature on the reaction yield and kinetics was also studied.
TypeArticle
URIhttp://hdl.handle.net/1822/1051
DOI10.1016/S0144-8617(98)00071-X
ISSN0144-8617
Peer-Reviewedyes
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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