Repositório Colecção: Livros (autor)
https://hdl.handle.net/1822/3714
Livros (autor)2024-03-29T15:51:00ZCell-derived matrices - Part B
https://hdl.handle.net/1822/66366
Título: Cell-derived matrices - Part B
Autor: Caballero, D.; Kundu, Subhas C; Reis, R. L.
Resumo: The cellular microenvironment has a fundamental regulatory role in major cellular functions. This microenvironment is made of an array of polymeric proteins and glycoproteins that constitute the extracellular matrix (ECM). The ECM does not serve only as a structural support but also as a bioactive material. This influences a large plethora of cellular (physiological and pathological) processes, such as differentiation, migration, or proliferation through both biochemical and mechanical stimuli. Many efforts have been dedicated in developing models of the ECM for the study of diverse varieties of physio-pathological phenomena; these models differ in their complexity, physiological relevance, and applications. Among all of them, cell-derived matrices (CDMs) have attracted significant attention due to their superior performance. CDMs are natural extracellular matrices produced by cells or derived from decellularized tissues. CDMs have been rapidly applied in cell biology as bioactive scaffolds due to their advanced capabilities to recapitulate the biological, biochemical, and mechanical properties of the native scenario. Cells cultured in CDMs display phenotypes, gene expression, and dynamic signatures similar to in vivo conditions. This improved performance has led to their adoption by the scientific community for the production of controlled cellular microenvironments for tissue engineering and regenerative medicine applications, disease modelling, or mechanistic studies, among others. Importantly, CDMs can be combined with other technologies typically employed in a cell biology lab, such as lab-on-a-chip devices, bioreactors, or engineered three-dimensional scaffolds for advanced biomimicry. In these two volumes of Methods in Cell Biology, we focus on the applications of CDMs to cell biology. Part A (Volume 156) deals with detailed methods describing the fabrication and engineering of CDMs, their compositional and/or structural analysis, and their application in biomedical studies. Part B (Volume 157) gives an account on the methods for the decellularization of tissues, their characterization, and application for tissue engineering and disease modelling. These two volumes cover a broad range of applications of CDMs to cell biology, with detailed discussions and examples. Overall, the purpose of these volumes is to provide to the readers a detailed outline of the methods available to fabricate, analyze, characterize, and understand the functional capabilities of CDMs for cell and molecular biology applications. We hope that these volumes will prove helpful in developing experiments to answer some of the important questions in cell biology, providing the readers with new interesting methods and insights to help them to reveal key mechanisms involved in physio-pathological processes.
<b>Tipo</b>: bookBiomimicked biomaterials
https://hdl.handle.net/1822/66326
Título: Biomimicked biomaterials
Autor: Chun, Heung Jae; Reis, R. L.; Motta, Antonella; Khang, Gilson
Resumo: This book is the second of two volumes that together offer a comprehensive account of cutting-edge advances in the development of biomaterials for use within tissue engineering and regenerative medicine. In this volume, which is devoted to biomimetic biomaterials, the opening section discusses bone regeneration by means of duckâ s feet-derived collagen scaffold and the use of decellularized extracellular matrices. The role of various novel biomimetic hydrogels in regenerative medicine is then considered in detail. The third section focuses on the control of stem cell fate by biomimetic biomaterials, covering exosome-integrated biomaterials for bone regeneration, cellular responses to materials for biomedical engineering, and the regulation of stem cell functions by micropatterned structures. Finally, the use of nano-intelligent biocomposites in regenerative medicine is addressed, with discussion of, for example, recent advances in biphasic calcium phosphate bioceramics and blood-contacting polymeric biomaterials. The authors are recognized experts in the interdisciplinary field of regenerative medicine and the book will be of value for all with an interest in regenerative medicine based on biomaterials.
<b>Tipo</b>: bookEncyclopedia of tissue engineering and regenerative medicine
https://hdl.handle.net/1822/62590
Título: Encyclopedia of tissue engineering and regenerative medicine
Autor: Reis, R. L.; Gomes, Manuela E.
Resumo: Encyclopedia of Tissue Engineering and Regenerative Medicine provides a comprehensive collection of personal overviews on the latest developments and likely future directions in the field. By providing concise expositions on a broad range of topics, this encyclopedia is an excellent resource. Tissue engineering and regenerative medicine are relatively new fields still in their early stages of development, yet they already show great promise. This encyclopedia brings together foundational content and hot topics in both disciplines into a comprehensive resource, allowing deeper interdisciplinary research and conclusions to be drawn from two increasingly connected areas of biomedicine.
<b>Tipo</b>: bookOsteochondral tissue engineering: nanotechnology, scaffolding-related developments and translation
https://hdl.handle.net/1822/58804
Título: Osteochondral tissue engineering: nanotechnology, scaffolding-related developments and translation
Autor: Oliveira, Joaquim M.; Pina, Sandra Cristina Almeida; San Roman, Julio; Reis, R. L.
Resumo: In the last few years, osteochondral tissue engineering has shown an increasing development in advanced tools and technologies for damaged underlying subchondral bone and cartilage tissue repair and regeneration. Considering the limitation of articular cartilage to heal and self-repair, new therapeutic options are essential to develop approaches based on suitable strategies made of appropriate engineered biomaterials. This book overviews the most recent developments in the field of osteochondral tissue engineering, and presents challenges and strategies being developed that face not only bone and cartilage regeneration, but also establish osteochondral interface formation, in order to translate it into a clinical setting. Topics embrace biomaterials advances in osteochondral tissue engineering, namely natural, synthetic, and bioceramics-based materials, nanotechnology approaches, as well as advanced processing methodology underlying tissue-engineered scaffolding development, such as 3D bioprinting, electrospinning, and supercritical fluid technology. Hydrogel systems for osteochondral applications are also detailed thoroughly. It also maximizes the reader insights into translational research and turning research into products, clinical trials and management of osteochondral lesions, and commercially available products. This is an ideal book for biomedical engineering students and a wide range of established researchers and professionals working in the orthopedic field.
<b>Tipo</b>: bookOsteochondral tissue engineering: challenges, current strategies, and technological advances
https://hdl.handle.net/1822/58803
Título: Osteochondral tissue engineering: challenges, current strategies, and technological advances
Autor: Oliveira, Joaquim M.; Pina, Sandra Cristina Almeida; San Roman, Julio; Reis, R. L.
Resumo: In the last few years, osteochondral tissue engineering has shown an increasing development in advanced tools and technologies for damaged underlying subchondral bone and cartilage tissue repair and regeneration. Considering the limitation of articular cartilage to heal and self-repair, new therapeutic options are essential to develop approaches based on suitable strategies made of appropriate engineered biomaterials. This book overviews the most recent developments in the field of osteochondral tissue engineering. It covers the concepts and current challenges for bone and cartilage repair and regeneration, along with technological advances for osteochondral tissue. Specific topics include viscosupplementation, tissue engineering approaches, technological advances with stem cells and cell-based therapies with applications for osteochondral, bioreactors and microfluidics including multi-chamber bioreactors, and in vitro and in vivo mimetic models. This book presents challenges and strategies being developed that face not only bone and cartilage regeneration, but also establish the osteochondral interface formation in order to translate it into a clinical setting. Each chapter is prepared by world know expert on their field, and serves as a core reference for biomedical engineering students and a wide range of established researchers and professionals working in the orthopedic field.
<b>Tipo</b>: book