http://hdl.handle.net/1822/1907 CDF_FAMO 2013-05-19T21:48:25Z http://hdl.handle.net/1822/24063 Title: A soft method for the production of CdSe and CdSe/ZnS quantum dots Authors: Coutinho, Paulo J. G. Abstract: A soft method for the production of CdSe and CdSe/Zns quantum dots is presented, as well as the main experimental results. Description: Comunicação Oral Convidada. 2013-05-01T00:00:00Z http://hdl.handle.net/1822/23604 Title: Comparison of IgA, TNF-α and surface tension of the tear film in two different times of the day Authors: Puinhas, A.; Sampaio, Paula; Castanheira, Elisabete M. S.; Oliveira, C. D. Real; Lira, Madalena Abstract: Purpose: The main goal was to study the biochemical composition of the tear film in two different times of the day. Methods: Tear samples were collected from 10 individuals, non contact lenses wearers, from the university population without pathologies. To assess daily variations in the tear film, samples were collected twice in the day, one early in the morning and another in the evening using capillary tubes. Tear protein profile was analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), Tumor necrosis factor α (TNF-α) and Immunoglobulin A (IgA) were quantified by Enzyme Linked ImmunoSorbent Assay (ELISA). Tear film stability was obtained through measurements of Non Invasive Break Up Time and tear surface tension was measured by obtaining the Langmuir isotherms. Results: The stability of the tear film was higher in the morning than in the afternoon; corresponding to a higher value of surface tension in the afternoon. Protein electrophoresis tear profile is variable during the day as IgA concentration decreased from morning to afternoon (p<0.05). TNF-α concentration also decreased, but there wasn’t significant statistical differences (p = 0.089) Conclusion: We concluded that there are daily variations in the composition and properties of the tear film, indicating that changes occur without being caused by contact lenses wear or by the presence of ocular and systemic pathologies. Presence of TNF-α isn’t a direct indication of inflammatory pathology, since this cytokine was identified in relatively high amounts in subjects without inflammatory pathology. 2013-01-01T00:00:00Z http://hdl.handle.net/1822/23329 Title: New 1,3-diarylureas linked by C-C Suzuki coupling to the methyl 3-aminothieno[3,2-b]pyridine-2-carboxylate moiety: synthesis and fluorescence studies in solution and in lipid membranes Authors: Queiroz, Maria João R. P.; Peixoto, Daniela; Rodrigues, Ana Rita O.; Mendes, Pedro M. F.; Costa, Cátia N. C.; Coutinho, Paulo J. G.; Castanheira, Elisabete M. S. Abstract: New six fluorescent 1,3-diarylureas linked by C-C Suzuki coupling to the 6-position of the methyl 3-aminothieno[3,2-b]pyridine-2-carboxylate moiety were prepared by reaction of the amino groups on the ortho or meta positions relative to the C-C bond of the Suzuki coupling products, with different para-substituted arylisocyanates (H, OMe, CN), in high to excellent yields. The fluorescence properties of the 1,3-diarylureas in solution and in lipid membranes of egg-yolk phosphatidylcholine (Egg-PC), dipalmitoyl phosphatidylcholine (DPPC), dipalmitoyl phosphatidylglycerol (DPPG) or dioctadecyldimethylammonium bromide (DODAB), with or without cholesterol (Ch), were studied. The six 1,3-diarylureas have reasonable fluorescence quantum yields in several solvents (between 0.02 and 0.69) and present a moderately solvent sensitive emission, but are not fluorescent in alcohols and water. The compounds bearing the arylurea moiety in the meta position relative to the C-C bond, especially with the OMe and CN substituents, present the better solvatochromic properties. Incorporation of the six compounds in lipid membranes indicates that all the compounds are deeply located in the hydrophobic region of the lipid bilayers, feeling the transition between the rigid gel phase and fluid phases. 2013-03-01T00:00:00Z http://hdl.handle.net/1822/23066 Title: Magnetoliposomes based on magnetite nanoparticles Authors: Castanheira, Elisabete M. S.; Mendes, Pedro M. F.; Coutinho, Paulo J. G.; Almeida, B. G.; Gomes, I.Tarroso; Araújo, J. P. Abstract: In this work, magnetic nanoparticles of magnetite were prepared by soft chemical methods, using different surfactants as templating media. These nanoparticles were either covered with a lipid bilayer, forming dry magnetoliposomes, or entrapped in liposomes - aqueous magnetoliposomes. 2013-02-01T00:00:00Z http://hdl.handle.net/1822/23065 Title: Magnetoliposomes based on nickel/silica core/shell nanoparticles Authors: Rodrigues, Ana Rita O.; Castanheira, Elisabete M. S.; Coutinho, Paulo J. G.; Almeida, B. G.; Gomes, I.Tarroso; Araújo, J. P. Abstract: In this work, magnetic nanoparticles of nickel core with silica shell were prepared by soft chemical methods, using tetraethyl orthosilicate (TEOS) and different surfactants as templating media. These nanoparticles were either covered with a lipid bilayer or entrapped in liposomes. 2013-02-01T00:00:00Z